Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola)

The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at -2 degrees C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and -6 degrees C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5 degrees C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up-regulated in both the -2 degrees C and -6 degrees C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up-regulated in the -6 degrees C animals.

Details

Publication status:
Published
Author(s):
Authors: Thorne, M.A.S., Worland, M.R., Feret, R., Deery, M.J., Lilley, K.S., Clark, Melody

On this site: Michael Thorne, Roger Worland, Melody Clark
Date:
1 January, 2011
Journal/Source:
Insect Molecular Biology / 20
Page(s):
303-310
Digital Object Identifier (DOI):
https://doi.org/10.1111/j.1365-2583.2010.01062.x