Metabolomic fingerprint of cryo-stress in a freeze tolerant insect

This study employed H-1 NMR spectroscopy to assay the metabolome of the high Arctic freeze-tolerant dipteran larvae, Heleomyza borealis, after recovery from exposure to a range of sub-zero temperature treatments. Our data demonstrate the resilience of freeze tolerance in individuals of this permanently freeze-tolerant species that were acclimated to summer temperatures (5 degrees C): recovery of homeostasis after 48h was not significantly disturbed by 2h exposures to -3, -12, or -20 degrees C. Evidence of homeostatic perturbation to cryo-stress - both in terms of changes in specific metabolite concentrations as well as systemic changes in metabolism determined using multivariate pattern recognition techniques - was expressed almost entirely at a temperature coincident with the significant onset of mortality (-25 degrees C) and considerably below the minimum winter temperatures of its over-wintering habitat (c.-12 degrees C).

Details

Publication status:
Published
Author(s):
Authors: Hawes, T.C., Hines, A., Viant, M.R, Bale, J.S., Worland, M. Roger, Convey, Peter

On this site: Roger Worland, Peter Convey
Date:
1 January, 2008
Journal/Source:
CryoLetters / 29
Page(s):
505-515